The Domestic Organic Investment Act of 2025 creates a new federal grant program to help American organic farmers and food businesses grow and compete better. Right now, the U.S. imports a lot of organic products, and many domestic organic producers struggle with outdated equipment, limited storage space, and difficulty getting their products to market. This bill establishes the Domestic Organic Investment Program, run by the U.S. Department of Agriculture, to provide grants that help eligible organic businesses upgrade their operations. The program supports two main types of projects: larger infrastructure projects (like building cold storage facilities or processing centers) that can receive up to $2 million in grants, and smaller equipment-only projects that can receive up to $100,000. Eligible recipients include certified organic farms, cooperatives, food processors, handlers, and tribal governments. The grants are competitive, meaning businesses must apply and explain how their project helps the organic supply chain. For larger projects, recipients must contribute at least 50% of the project cost themselves, while equipment-only projects require a 25% match—though the USDA can reduce these requirements for beginning farmers, ranchers, and veterans. The program also provides technical assistance to help businesses succeed. Overall, this is designed to strengthen America's domestic organic food system by removing bottlenecks and helping producers invest in modern infrastructure.